Granulin anticorps

N° du produit ABIN325602

Cet anticorps anti-Granulin est un anticorps Souris Monoclonal détectant Granulin dans WB, IP et ELISA. Adapté pour Humain.

Aperçu rapide pour Granulin anticorps (ABIN325602)

Antigène: Granulin (GRN)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Granulin est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA

Détail du produit anti-Granulin anticorps

Specificité: Recognizes human GRN. In direct ELISAs and Western Blots this antibody shows no cross-reactivity with rmProgranulin.

Purification: Protein G purified

Immunogène: NS0-derived rhGRN.

Type of Immunogen: Recombinant protein

Isotype: IgG2a

Information d'application

Indications d'application: Approved: ELISA (0.5 - 1 μg/mL), IP (25 μg/mL), WB (1 - 2 μg/mL)

Usage: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Direct ELISA: 0.5-1.0 μg/mL, The detection limit for rhGRN is approximately 2 ng/well. Western Blot: 1-2 μg/mL, Using a colorimetric detection system, the detection limit for rhGRN is ~5 ng/lane under non-reducing conditions. Use under reducing conditions is not recommended. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Immunoprecipitation: 25 μg/mL to immunoprecipitate rhGRN from conditioned cell culture medium. The recovered rhGRN can be detected in Western Blots using an affinity-purified polyclonal antibody.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 200 μL sterile PBS

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 8.0, 20  mM Tris, 300  mM sodium chloride, 5 % trehalose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: Short term: -20°C
Long term: -20°C.

Détails sur Granulin

Antigène: Granulin (GRN)

Autre désignation: GRN / Progranulin / Granulin

Sujet: Name/Gene ID: GRN
Family: Hormone

Synonyms: GRN, Acrogranin, CLN11, Epithelin precursor, GEP, gp88, Granulin, Granulin-epithelin, Granulins, PC cell-derived growth factor, PCDGF, PEPI, PGRN, Proepithelin, Progranulin

ID gène: 2896

UniProt: P28799